Mitigation of aflatoxin B₁ in fish feed by peroxidase from soybean meal

Abstract

Potential of the enzyme peroxidase (PO) from soybean meal to mitigate aflatoxin B₁ (AFB₁) in fish feed was evaluated. Reaction parameters studied in the wet stage of the feed production process were enzyme activity (0.01–0.1 U/g), temperature (20–36 °C), time (0–8 h) and humidity content (40–70%). Feed was produced in conformity with the National Research Council and spiked with AFB₁ at 10 ng/g. Any residual concentration of AFB₁ in the diet was extracted by the QuEChERS method and quantified by a liquid chromatograph with a fluorescence detector. AFB₁ mitigation of 90% was reached when feed production conditions were 0.035 U/g, 32 °C, 6 h and 70% humidity. Therefore, application of PO to the feed industry may be considered a promising tool for mitigation of AFB₁, considering its toxicity and frequent occurrence. In addition, it guarantees safe food for consumers of fish farming products, as AFB₁ can bioaccumulate in the food chain. It also provides an alternative use for soybean meal that would previously be discarded.

Keywords:

• Enzyme
• degradation
• mycotoxin
• oxidoreductase
• pisciculture

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was partially funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001 and CAPES-PrInt Program under Grant #88887.310848/2018-00.