Hexavalent chromium reduction by Escherichia coli in the presence of ferric iron

Abstract

The potential of Cr(VI) reduction by Escherichia coli in the presence of soluble Fe(III) was investigated to explore the chemo-biologically mediated reduction process under anaerobic condition. The reduction efficiency of Cr(VI) reached 95% within 24 h. The influences of experimental parameters, including initial pH, temperature, Fe(III) dosage, carbon source, and chelating agent, were also investigated. The highest efficiency of reduction was observed when pH was 5.8 and temperature was 32°C. Amendments of culture medium with Fe(III) and citric-3Na enhanced Cr(VI) reduction, while the addition of EDTA-2Na inhibited the process. Analysis showed that soluble Fe(III) enhanced the reduction process by shuttling electrons from bio-reduced Fe(II) to Cr(VI) in a coupled biotic-abiotic cycle and hence, Cr(VI) was reduced to Cr(III) followed by deposition to sludge.

Keywords:

• Hexavalent chromium
• Microbial reduction
• Optimum reduction
• Ferric iron

Acknowledgments

The authors extend their gratitude for the financial support provided by the Zhejiang Provincial Natural Science Foundation of China (No. R5090033) and the PhD Programs Foundation of Ministry of Education of the P.R. China (200803350077).