![Sample our Engineering & Technology journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5933/TOC-Subject-Sample-2021-Engineering-Tech.jpg"})
Abstract
In the present context of wastewater treatment, biodegradation-based techniques are well known as a low-cost and environment friendly approach, for cleaning up various agro effluents. Likewise, the efficiency of indigenous Staphylococcus aureus strain 502 has been investigated in the study for removal of toxic insecticide acetamiprid (ACE) from wetland wastewater. The isolation and enrichment was done in minimal salt media enhanced with 50 mg L−1 of ACE as sole carbon, nitrogen, and energy source, incubated at 35°C in 100 rpm for about 5 d. Central composite design (CCD) was applied to predict the optimal condition of ACE removal. The correlation of the operating variables and the response based on CCD were fitted to quadratic equation. The mathematical model fitting of growth curve of the isolated bacteria was also studied. The strain S. aureus 502A was observed to have maximum consumption of 61.68% of ACE in 24 h, analyzed using GC–MS. The rate kinetic analysis was studied using zero-order and three half-order kinetic models. Among the identified metabolites, Benzothiazole, 2-(2-hydroxyethylthio) with an effective peak at retention time 7.00 min was observed to be the end product of ACE biodegradation. Further, toxicological analysis on Bacillus subtilis exhibited no inhibition zone, suggesting the non-toxic nature of the degraded metabolite.
Acknowledgment
The authors acknowledge Indian Association for Cultivation of Science for assistance in FESEM analysis and Bio-Axis DNA Research Centre (Pvt.) Ltd. Hyderabad, India for identification of the isolated bacterial strain using 16S-rRNA technique. The authors are grateful to the University Grants Commission (UGC) UPE Phase II for their financial support to carry out the present work. The authors also acknowledge the contribution of UGC under Indo-Norwegian Cooperation Program 2014(INCP) (vide sanction letter no. 58-3/2014(IC) dated December 26, 2014).