ABSTRACT
Adipose tissue is highly dynamic and increases its size dependent on the status of nutrition. Generally, an increase of adipose tissue mass is attributed to two mechanisms, namely hypertrophy (increase in adipocyte size) and hyperplasia (increase in adipocyte number). Here, we analyzed the proliferation capacity of a pool of nutrition sensing preadipocytes after short-term high fat diet (HFD) feeding. We show that this process is age independent and that adipocyte hyperplasia seems not to be dependent on adipocyte hypertrophy. Further, we could show that the subsequent development into adipocytes is influenced by the duration of HFD feeding after proliferation. Our data also demonstrate that the studied pool of preadipocytes seems to be finite and cannot be reactivated by multiple bouts of HFD feeding. In conclusion, our results indicate an important link between stem cells, nutrition status and homeostasis in the epididymal adipose tissue.
Acknowledgments
We thank E. Kiehlmann for her help with the microscopy sections. Also we thank D. H. Dapito for her constructive discussions and carefully reading the manuscript. We thank G. Grandl for the Macro to calculate adipocyte area.
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The authors declare that they have no conflict of interest.
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This article has been republished with minor changes. These changes do not impact the academic content of the article.
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Notes on contributors
Elisabeth Kulenkampff
Elisabeth Kulenkampff and Christian Wolfrum designed the study and wrote the manuscript. EK preformed all experiments.