Regulatory T cells do not suppress rather activate human basophils by IL-3 and STAT5-dependent mechanisms

ABSTRACT

Basophils play an important role in orienting Th2 immune response, and in the pathogenesis of allergic and inflammatory disorders. However, the mechanism by which basophils are kept in check remains unclear and hence we explored the role of regulatory T cells (Treg cells) in this process. We demonstrate that human Treg cells do not suppress rather induce activation of basophils, and promote Th2 responses by IL-3 and STAT5-dependent mechanism.

KEYWORDS:

• Basophil
• CD4⁺CD25⁺FoxP3⁺ regulatory T cell
• cancer
• immune evasion; Tregs; Th2 response

Abbreviations

CCL17	=	Chemokine (C-C motif) ligand 17
CTLA-4	=	Cytotoxic T lymphocyte antigen-4
CXCL9	=	C-X-C Motif Chemokine Ligand 9
IL-3R	=	IL-3 receptor
IL-10	=	Interleukin 10
LAG-3	=	Lymphocyte-activation gene-3
NK cells	=	Natural killer cells
PDAC	=	Pancreatic ductal adenocarcinomas
Treg cells	=	Regulatory T cells
STAT5	=	Signal transducer and activator of transcription 5
TGF-β1	=	Transforming growth factor beta 1

Acknowledgments

We thank all the lab members and collaborators who contributed to the data discussed in this article.

Disclosure of potential conflicts of interest

No potential conflicts of interest were disclosed.

Additional information

Funding

Supported by the Institut National de la Santé et de la Recherche Médicale (INSERM), Sorbonne Université, Université de Paris, Agence Nationale de la Recherche, France (ANR-19-CE17-0021(BASIN)).