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Abstract
Our understanding of the mechanisms of effect of Ginkgo Seed Polysaccharides (GSPs) on the immune system remains unclear. The aim of this work was to investigate the effect of GSPs on the maturation and function of bone-marrow-derived dendritic cells (BMDCs). The results demonstrate that GSP could exert positive immune modulation on the maturation and functions of BMDCs. This effect was evidenced by decreased changes of phagosome number inside BMDCs, decreased activity of acidic phosphatase (ACP), decreased phagocytosis of BMDCs, and increased changes of key membrane molecules on BMDCs. Upregulated production of cytokines IL-12 and TNF-α also was confirmed. Therefore, it can be concluded that GSPs can efficiently induce the maturation of BMDCs. Our exploration provides direct data and a rationale for potential application of GSPs as an immune enhancer in improving immunity and as a potent adjuvant in the design of DC-based vaccines.
Abbreviations:
- BMC-Bone marrow cell
- BMDCs-Bone marrow derived dendritic cells
- DCs-dendritic cells
- GSPs-Gingko seed polysaccharides
- IL-4-interleukin-4
- GM-CSF-Granulocyte macrophage colony stimulating factor
- FCM-Flow cytometry
- MACS-Magnetic activated cell sorting
- PBS-Phosphate buffer solution
- ACP-Acidic phosphatase
- APCs-Antigen presenting cells
- LPS-Lipopolysaccharide
- TEM-Transmission electron microscopy
- MTS-methyl tolyl sulfide
- ELISA-Enzyme linked immunosorbent assay
Disclosure of Potential Conflicts of Interest
No potential conflicts of interest were disclosed.
Funding
This study was supported by the funding from project for construction of major discipline platform in universities of Liaoning province and funding from Liaoning science foundation (2009225008-7, 2012225016).