Allogenic mouse cell vaccine inhibits lung cancer progression by inhibiting angiogenesis

ABSTRACT

Aim: This research investigated the therapeutic effect of an allogeneic mouse brain microvascular endothelial cell vaccine on lung cancer and further elucidated its potential anti-angiogenic mechanism.

Materials & methods: The immune effect of the allogeneic bEnd.3 vaccine and DC vaccine loaded with bEnd.3 antigen on the subcutaneous transplantation of Lewis lung cancer (LLC) was assessed by ELISA, the CCK test and the CTL killing test. The mechanism was preliminarily revealed by immunohistochemistry and immunoblot analysis.

Results: This study revealed that tumor volume was decreased (p < .01) and the survival was prolonged significantly (p < .05) by the bEnd.3 vaccine in subcutaneous LLC transplantation in the vaccine prevention group. In contrast, both tumor volume in the serum therapeutic group and survival of bEnd.3 vaccine were not significantly different from those of the control group (p > .05). Importantly, tumor volume and survival of the T lymphocyte therapeutic group were decreased and prolonged (p < .05). In addition, both tumor volume and survival of DC vaccine loaded with bEnd.3 in the vaccine prevention group were decreased and prolonged significantly (p < .01). Furthermore, bEnd.3 vaccine and DC vaccine loaded with bEnd.3 both produced the activity of killing bEnd.3 target cells in vitro.The reason may induce the immune mice to produce anti-VEGFR-II, anti-endoglin and anti-integrin αν antibodies to have an anti-angiogenesis function.

Conclusion: The allogeneic mouse bEnd.3 cell vaccine can block angiogenesis and prevent the development of lung cancer transplantation tumors.

KEYWORDS:

• Vaccine
• allogeneic
• vascular endothelial cells
• anti-angiogenesis
• dendritic cells
• immunotherapy

Acknowledgments

N/A

Author contributions

JZ, JMZ, and JL conceived and designed the study. JZ and XLZ performed the experiments. YYD and LRZ collected and analysed the data. JZ wrote the original manuscript. ZMD, JMZ, and JL reviewed and edited the manuscript. All authors have read and approved the current manuscript.

Data sharing statement

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Ethical disclosure

All the protocols described in the present study were reviewed and approved by the Ethics Committee of Zhengzhou University (Zhengzhou, Henan, China).

Information pertaining to writing assistance

Writing assistance from American Journal Experts was utilized in the production of this manuscript, which was funded by the Natural Science Foundation of China (81572972) and the Supporting Plan of Scientific and Technological Innovation Team in Universities of Henan Province (20IRTSTHN029).

Additional information

Funding

The study was supported by the Natural Science Foundation of China [81572972] and the Supporting Plan of Scientific and Technological Innovation Team in Universities of Henan Province [20IRTSTHN029].