Quantitative characterization of the T cell receptor repertoires of human immunized by rabies virus vaccine

ABSTRACT

Cellular immunity is crucial for an efficient host immune response against rabies virus (RABV) infection. But the T cell receptor (TCR) repertoire in human after RABV vaccine immunization remained unclear. In this study, we conducted high-throughput sequencing of TCR β chain complementarity determining region 3(CDR3) repertoires in 4 healthy volunteers before and after immunization with RABV vaccine. Our data showed that RABV vaccination changed the TCR diversity and the usage of V/J gene segments, as well as V-J pairing. The high-frequency clonotypes that altered after vaccination were identified. These results may provide us with new insights into T cell receptor condition after RABV vaccination.

KEYWORDS:

• Rabies virus (RABV)
• T cell receptor (TCR) repertoire
• high-throughput sequencing
• complementarity determining region 3(CDR3)
• immune response

Disclosure of potential conflicts of interest

No potential conflicts of interest were disclosed.

Additional information

Funding

This study was supported by The National Key Research and Development Program of China (国家重点研发计划) [Grant No.: 2016YFD0500405 to Dr. Pingsen Zhao], The National Key Research and Development Program of China [Grant No.: 2017YFD0501705 to Dr. Pingsen Zhao] and Natural Science Foundation of Guangdong Province, China [Grant No.: 2016A030307031 to Dr. Pingsen Zhao].