MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

ABSTRACT

Previous studies reported that miR-330-3p was involved in the progression of several cancers, but the potential roles of miR-330-3p in ovarian cancer (OC) were unclear. In the current study, we aimed to explore the expression pattern and functions of miR-330-3p in OC. The expression level of miR-330-3p in OC tissues and cell lines was detected using RT-qPCR. The proliferation, migration and invasion of OC cells were detected using CCK-8 assay and transwell assay, respectively. Bioinformatics analysis and luciferase reporter assay were used to analyze the targeted binding site of miR-330-3p and RIPK4. The results showed that miR-330-3p was significantly downregulated in OC tissues and cell lines. Overexpression of miR-330-3p inhibited the proliferation, migration and invasion of OC cells. Mechanistically, a dual-luciferase reported assay showed that RIPK4 is a target gene of miR-330-3p. Furthermore, rescue experiments revealed that miR-330-3p suppressed the proliferation, migration and invasion of OC cells by targeting RIPK4. In summary, our findings indicated that miR-330-3p suppressed the progression of OC by targeting RIPK4. Our results indicated that miR-330-3p/RIPK4 axis might act as a novel therapeutic target for OC treatment.

Graphical abstract

KEYWORDS:

• Mir-330-3p
• RIPK4
• ovarian cancer

Highlights

1. miR-330-3p was downregulated in OC tissue specimens and cell lines.

2. Overexpression of miR-330-3p suppressed the proliferation, migration and invasion of OC cells.

3. Overexpression of miR-330-3p suppressed the proliferation, migration and invasion of OC cells via RIPK4.

Funding

This research was supported by the National Natural Science Foundation of China, (81860284, 31960146) and Provincial Natural Science Foundation of Jiangxi (20181BAB205024, 20192BAB205017).

Disclosure statement

All the authors had no conflicts of interest or financial ties to disclose.