Interleukin IL-5 alleviates sepsis-induced acute lung injury by regulating the immune response in rats

ABSTRACT

To study the effect of IL-5 on the immune response and lung injury in rats with sepsis. We constructed a rat model of sepsis by cecal ligation and puncture (CLP). The rats were randomly divided into the control group, the sham group, the CLP group and the IL-5 group, with 6 rats in each group. With the induction of CLP, the lung tissue of rats was severely injured, and the water content of lung tissue was significantly increased. Moreover, the ratio of CD4⁺/CD8⁺ was significantly decreased and Th1/Th2 was significantly increased in the peripheral blood. The content of IL-6, TNF-α, and HMGB1 was found to be increased in the CLP group. However, with the injection of IL-5, the degree of lung tissue injury in CLP rats was alleviated and the water content of lung tissue was significantly reduced. The ratio of CD4⁺/CD8⁺ was increased and Th1/Th2 was significantly down-regulated in the peripheral blood and the levels of IL-6, TNF-α, and HMGB1 in serum were significantly decreased. In conclusion, IL-5 can alleviate lung injury by regulating the immune response and inhibiting the systemic inflammatory response induced by sepsis.

Graphical abstract

KEYWORDS:

• Sepsis
• acute lung injury
• immune response
• il-5

Highlights

1. IL-5 alleviates CLP-induced lung injury in rats.

2. IL-5 reduces the water content in the lung tissue of CLP rats.

3. IL-5 increases CD4⁺/CD8⁺ ratio and decreases Th1/Th2 ratio of CLP rats.

4. IL-5 decreases the expression of inflammatory cytokines of CLP rats.

Disclosure Statement

The authors declare that they have no competing interests.