Circular RNA Circ_0000677 promotes cell proliferation by regulating microRNA-106b-5p/CCND1 in non-small cell lung cancer

ABSTRACT

Recently, circular RNAs (circRNAs) have become an intense focus of research and large numbers of circRNAs have been identified, awaiting functional elucidation. Thus, the present study aims to examine the regulation of circRNAs and its molecular mechanism in lung cancer growth. Here, we show that circular RNA circ_0000677 was overexpressed and correlated with poor prognosis in non‐small cell lung cancer (NSCLC) patients. Functionally, circ_0000677 knockdown markedly inhibited proliferation of NSCLC cells by observing of immunofluorescence staining of Ki67, clone formation assay, and xenograft experiments. In mechanism, circ_0000677 acted as a sponge of microRNA-106b and further regulated CCDND1 gene expression in NSCLC cells by dual luciferase activity assay and their expression examination. Taken together, these findings suggest a role for circ_0000677/miR-106b/CCND1 regulation axis in promoting NSCLC growth and progression.

KEYWORDS:

• Circ_0000677
• miR-106b-5p
• ccnd1
• proliferation
• non-small cell lung cancer

Disclosure statement

No potential conflict of interest was reported by the author(s).

Highlight

• Circ_0000677 expression is elevated in NSCLC tissues than that in adjacent noncancerous tissues.

• Circ_0000677 directly targets miR-106b and regulates the cell cycle regulator CCND1.

• Circ_0000677 expression can predict overall survival in patients with NSCLC.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.