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ABSTRACT
Clear cell renal cell carcinoma (ccRCC) is an aggressive malignancy with a poor prognosis. Therefore, investigating the molecular mechanism of ccRCC is important for ccRCC treatment. Here, we aimed to explore the effect of the long non-coding RNA ARAP1-AS1/miR-361-3p/PGF axis on ccRCC. The expression of lncRNA ARAP1-AS1, miR-361-3p, and placental growth factor (PGF) in ccRCC cells was verified by real-time quantitative PCR (RT-qPCR). The influence of the ARAP1-AS1/miR-361-3p/PGF axis on ccRCC cells was identified using the Cell Counting Kit-8 (CCK-8) assay, colony formation assay, flow cytometry, and wound healing assay. The interaction between ARAP1-AS1, miR-361-3p, and PGF was confirmed by bioinformatics analysis and luciferase assay. The results showed that the levels of ARAP1-AS1 and PGF increased in ccRCC cells, while miR-361-3p expression decreased. Cell functional experiments showed that cell proliferation and migration were inhibited by silencing ARAP1-AS1 or PGF, while miR-361-3p inhibitor or PGF overexpression could relieve the inhibitory effect of silencing ARAP1-AS1 on ccRCC cells. Moreover, ARAP1-AS1 sponges miR-361-3p to increase PGF expression. In conclusion, our study revealed that ARAP1-AS1 enhanced the malignancy of ccRCC cells by regulating the miR-361-3p/PGF axis.
Highlights
1. ARAP1-AS1 expression is upregulated in clear cell renal cell carcinoma (ccRCC).
2. ARAP1-AS1 promoted cell proliferation and cell migration in ccRCC.
3. ARAP1-AS1 facilitated the malignancy of ccRCC cells by sponging the miR-361-3p/PGF axis.
Availability of data and material
The data used to support the findings of this study are available from the corresponding author upon request.
Ethics approval and informed consent
The present study was approved by the Ethics Committee of Hubei Hospital of Integrated Traditional Chinese and Western Medicine. The processing of clinical tissue samples is in strict compliance with the ethical standards of the Declaration of Helsinki. All patients signed written informed consent.
Consent for publication
Consent for publication was obtained from the participants.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Author contributions
LPZ designed this study, carried out the experiments, interpreted the data, and wrote and drafted the manuscript; XWZ performed the data analysis, reviewed and edited the manuscript.
Supplementary material
Supplemental data for this article can be accessed here.
