Long non-coding RNA LINC01270 is an onco-promotor in lung adenocarcinoma by upregulating LARP1 via sponging miR-326

ABSTRACT

Accumulating evidence have proved the key role of long non-coding RNA in lung adenocarcinoma (LUAD) progression. Bioinformatics analysis is used to seek the differentially expressed lncRNA LINC01270 from TCGA database. The overexpression of LINC01270 was then verified in LUAD tumor tissues and cell lines by qRT-PCR. LINC01270 knockdown resulted in impaired cell proliferative and invasive ability via CCK-8 assay, EdU assay, colony formation assay, transwell assay, while aberrant upregulation of LINC01270 led to enhanced cell growth and invasion. Moreover, LINC01270 was found inhibiting miR-326 and thereby overexpressing the abundance of LARP1 to promote LUAD development via PI3K/AKT pathway. It was also proved that LINC01270 knockdown could suppress LUAD tumor growth in vivo. All of these findings demonstrate thatLINC01270 is a tumor promotor in LUAD via enhancing LARP1 expressed by sponging miR-326 to facilitate the development of LUAD. LINC01270 play a significant role in LUAD, which could serve as biomarkers for early diagnosis and a novel targeted remedy.

GRAPHICAL ABSTRACT

KEYWORDS:

• LINC01270
• miR-326
• LARP1
• lung adenocarcinoma

Disclosure statement

No potential conflict of interest was reported by the author(s).

Author contributions

Weiran Zhang, Cheng Cao, and Jingfu Shen conducted these experiments and wrote the draft. Shaoyin Shan and Yuanhao Tong analyzed the data. Hongyan Cai and Zhifeng Han performed parts of the experiments. Huiping Chai supervised this study.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/21655979.2022.2090183

Additional information

Funding

This study was supported by Foundation of Anhui Medical University under Grant No. 2019xkj148.