Comparative metabolomics analysis of pericarp from four varieties of Zanthoxylum bungeanum Maxim

ABSTRACT

A qualitative and quantitative analysis of metabolites was performed by metabolomics comparation on the pericarps of four varieties of Zanthoxylum bungeanum Maxim. The Zanthoxylum bunganum as scion combined with three rootstock varieties of Zanthoxylum piasezkii Maxim (YJ), July Zanthoxylum bunganum Maxim (QJ), and August Zanthoxylum bunganum Maxim (BJ), at the same time Zanthoxylum bungeanum seedlings breeding were compared as control (MJ). A total of 1429 metabolites were identified in Zanthoxylum bungeanum Maxim pericarps based on chromatography and mass spectrometry dual detection platform. While the metabolites between four varieties of Z. bungeanum varied, there was identified 31, 15, 7, 79, 42, 19 down-regulated and 55, 50, 13, 75, 43, 27 up-regulated differential metabolites between MJ and BJ, MJ and QJ, MJ and YJ, QJ and BJ, YJ and BJ, YJ and QJ. Meanwhile, the differential metabolites composition was distinct among various varieties of Z. bungeanum and dominant by phenolic compounds flavonoid and phenolic acids, especially highest in varieties July Zanthoxylum bunganum Maxim. Highlight A comparative metabolomics analyzed in four varieties of Zanthoxylum bungeanum pericarp.Total 1429 metabolites were identified and mainly in flavonoid and phenolic acid.July and August Zanthoxylum bunganum Maxim has highest antioxidant capacity.The rootstock July Zanthoxylum bunganum Maxim was recommended in Loess Plateau.

Graphical abstract

KEYWORDS:

• Metabolomics
• different metabolites
• Zanthoxylum bungeanum Maxim
• rootstock variety

Novelty statement

This study qualitative and quantitative analysis of metabolites on the pericarps of four varieties of Zanthoxylum bungeanum Maxim by metabolomics comparative. Rootstock selection strongly effects the pericarp metabolite profile of Z. bungeanum varieties. The primarily for polyphenols included flavonoids and phenolic acids were predominant compounds. Overall, the metabolism of rootstock July Zanthoxylum bunganum Maxim and scion Zanthoxylum bunganum combination was activated in physiological, biochemical and molecular adaptations, contributed to highest antioxidant capacity and stress resistance to response the unique natural environment of the Loess Plateau.

Acknowledgements

The authors are grateful for the financial support from Central Forestry Science and Technology Promotion Demonstration Project ([2020] ZYTG07), Gansu Provincial Science and Technology Project (20YF8NK161), 2017 Longyuan Youth Innovation and Entrepreneurial Talent Individual Project, and Longnan Science and Technology Project 2022-S·SZ-06. We are also thanks to our all laboratory colleagues and research staff members for their constructive advice and help.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the [Funding Agency #1 Forestry Science and Technology Promotion Demonstration Project] under Grant [2020 ZYTG07], [Funding Agency #2 ] under Grant [20YF8NK161], and [Funding Agency #3 2017-Longyuan Youth Innovation and Entrepreneurial Talent Individual Project Longnan Science and Technology Project] under Grant [2017 Longyuan Youth Innovation and Entrepreneurial Talent Individual Project and Longnan Science and Technology Project 2022-S·SZ-06; Gansu Provincial Science and Technology ProjectFinancial support from Central Forestry Science and Technology Promotion Demonstration Project [[2020]ZYTG07.