Construction of a stable w/o nano-emulsion as a potential adjuvant for foot and mouth disease virus vaccine

Abstract

A stable and bio-compatible w/o nano-emulsion was developed as adjuvant for foot and mouth disease (FMD) vaccine with isopropyl myristate as oil phase, polyglycerol polyricinoleate, and Tween 80 as surfactants. Dissolving Tween 80 in aqueous phase decreased the droplet size and improved stability of the emulsion through narrowing size distribution, increasing viscosity, and reducing the interfacial tension between oil and water. The nano-emulsion was then formulated with inactivated FMD virus and ovalbumin to investigate its potential application as an adjuvant. Compared with commercial ISA-206 adjuvant, the novel w/o nano-emulsion was able to induce both humoral- and cell-mediated immune response.

Keywords:

• Adjuvant
• foot-and-mouth disease vaccine
• polyglycerol polyricinoleate
• stability
• w/o nano-emulsion

Acknowledgements

We gratefully acknowledge LDS Technology Limited Company (Beijing, China) for their assistance in the emulsions global stability measurements.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

Funding information

We are grateful for the financial support from the National Natural Science Foundation of China (21336010), the National High Technology Research and Development Program of China (863 Program, 2014AA021005, 2014AA021006), and National Science and Technology Major Project (2013ZX10004606).