Screening of process variables using Plackett–Burman design in the fabrication of gedunin-loaded liposomes

Abstract

This study is to screening the formulation and process variables that produce significant effect on the gedunin-loaded liposome formulations by using quality-by-design approach. Placket–Burman screening design was used to screen the most influencing formulation and process variables. Mean vesicle size, zeta potential, entrapment efficiency, and loading capacity were found in the range of 112–990 nm, −19.39 to −39.20 mV, 45.25–87.60%, and 3.54–10.47%, respectively. Differential scanning calorimetry (DSC) and powder X-ray diffraction (XRD) result suggested that Gedunin encapsulated within liposome as amorphous state. The analysis of Pareto chart represented that selected independent variables had a most significant effect on dependent variables.

Keywords:

• Gedunin
• liposome
• Plackett–Burman design
• quality by design

Acknowledgements

The authors are thankful to Director, University Institute of Pharmacy, Pt Ravishankar Shukla University Raipur, (C. G.) for providing necessary infrastructural facilities. One of the authors Anil Kumar Sahu is thankful to UGC-BSR for JRF and extends her gratitude towards the supervisor for guidance and support. Author also wants to thank the library of Pt. Ravishankar Shukla University for providing e-resources available through UGC-INFLIBNET.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.