Optimization of desolvation process for fabrication of lactoferrin nanoparticles using quality by design approach

Abstract

The present work deals with the design and process optimization for preparation of lactoferrin nanoparticles as carrier for delivery of curcumin (CLf-NPs) using quality by design (QbD) approach. Desolvation technique was selected for preparation of Lf-NPs. The concept of QbD was followed in stepwise manner including risk analysis FMEA methodology. Plackett–Burman screening design employing eight factors and two levels was selected for screening study and custom design was selected for further analysis. Curcumin was used as model polyphenol for assessing the encapsulating efficiency of Lf-NPs.

Keywords:

• Critical quality attributes
• desolvation
• lactoferrin
• optimization
• robustness

Acknowledgements

The authors are thankful to Frieslandcampina, the Netherlands, for generously gifting sample of lactoferrin, ACTREC, Mumbai (India) and SAIF, Indian Institute of Technology, Mumbai for carrying out the in vitro cell viability assay and TEM analysis. Authors are also thankful to HRPIPER and North Maharashtra University, Jalgaon, for providing necessary facilities for carrying out the research effectively.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.