Upregulation of miR-9 and Let-7a by nanoencapsulated chrysin in gastric cancer cells

Abstract

Chrysin, as a flavone, has showed cancer chemopreventive activity. The present study utilized the PLGA–PEG–chrysin to evaluate the expression of miR-9 and Let-7a in human gastric cells. The structure of nanoparticles and encapsulated chrysin was evaluated using ¹H NMR, FT-IR, and SEM. MTT assay was used for the evaluation of cytotoxicity effect of nanoencapsulated chrysin. Expression levels of miR-9 and Let7-a were studied by real-time PCR. The results demonstrated that chrysin–PLGA–PEG nanoparticles are more effective than pure chrysin in upregulation of miR-9 and Let-7a due to enhanced uptake by cells. Therefore, PLGA–PEG could be a superior carrier for this kind of hydrophobic agent.

Keywords:

• PLGA–PEG–chrysin
• miR-9
• Let7-a
• real-time PCR
• gastric cancer

Disclosure statement

The authors declare that they have no conflict of interest.

Funding

The authors would like to thank Hematology and Oncology Research Center, Tabriz University of Medical Sciences for supporting this project [grant no.: 92/34].