The cytoprotective role of omentin against oxidative stress-induced PC12 apoptosis

Abstract

Oxidative stress has been proven to play a critical role in the pathogenesis of neuronal injury. As a novel adipocytokine, omentin is produced by visceral adipose with insulin sensitizing effects and has been revealed to possess anti-inflammatory effects. However, the possible effect of omentin on oxidative stress remains unknown. The present study aimed to detect the potential protective effect of omentin against hydrogen peroxide (H₂O₂)-induced cytotoxicity of PC12 cells. The results showed that no cytotoxic effect was shown in PC12 cells co-cultured with omentin alone at a concentration of 50–1000 ng/mL. The CCK8 and TUNEL assays suggested that omentin could remarkably attenuate apoptosis induced by 100 μM H₂O₂. The PCR and western blotting showed that the expression levels of Bax was significantly inhibited by omentin via the upregulation of miR-128-3p at its 3′-UTR. Taken together, these results indicated that omentin protects PC12 cells against H₂O₂-induced apoptosis, and further studies need to be conducted before utilization in the clinic for the treatment of neurodegenerative diseases.

Keywords:

• Omentin
• oxidative stress
• hydrogen peroxide
• PC12

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The datasets generated and/or analyzed during the present study are available from the corresponding author upon reasonable request.

Additional information

Funding

The present study was supported by the National Natural Science Foundation of China [81660210], Science and Technology Project of Xi’an, Shaanxi Province, China [201805104YX12SF38(2)] and Key Research and Development Program of Shaanxi [2020ZDLSF04-03].