Anti-microbial and anti-cancer activities of Mn_0.5Zn_0.5Dy_xFe_2-xO₄ (x ≤ 0.1) nanoparticles

Abstract

Combining two or more nanoparticles is a promising approach. Previously we have reported synthesis of nanoparticles Dysprosium (Dy) substituted with manganese (Mn) zinc (Zn) by using ultrasonication method. The five different nanoparticles (NPs) Mn_0.5Zn_0.5Dy_xFe_2-xO₄ (x ≤ 0.1) have been structurally and morphologically characterized but there is no report on the biological application of these NPs. In the present study, we have examined the anti-cancer, anti-bacterial, and anti-fungal activities of Mn_0.5Zn_0.5Dy_xFe_2-xO₄ (x ≤ 0.1) NPs. Human colorectal carcinoma cells (HCT-116) were tested with different concentrations of NPs by using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. In addition, the impact of NPs was also examined on normal cells such as human embryonic kidney cells, HEK-293. After 48 h of treatment, Mn_0.5Zn_0.5Dy_xFe_2-xO₄ NPs (x = 0.02, 0.04 and 0.06) showed no inhibitory action on cancer cell’s growth and proliferation, whereas Mn_0.5Zn_0.5Dy_xFe_2-xO₄ NPs (x = 0.08 and 0.1) showed profound inhibitory action on cancer cell’s growth and proliferation. However, the treatment of Mn_0.5Zn_0.5Dy_xFe_2-xO₄ NPs on the normal cells (HEK-293) did not show cytotoxic or inhibitory action on HEK-293 cells. The treatment of Mn_0.5Zn_0.5Dy_xFe_2-xO₄ NPs (x ≤ 0.1) also inhibited both the bacteria (Escherichia coli ATCC35218 and Staphylococcus aureus) with lowest MIC and MBC values of 4 and 8 mg/mL and fungus (Candida albicans) with MIC and MFC values of 4 and 8 mg/mL on treatment with x = 0.08 and 0. 1.

Keywords:

• Nanoparticles
• spinel ferrites
• anti-cancer
• anti-bacterial
• anti-fungal
• in vitro assay

Authors consent

All authors have approved the final version of the manuscript.

Acknowledgements

Authors highly acknowledged the Deanship of Scientific Research (DSR) and Institute for Research & Medical Consultations (IRMC) of Imam Abdulrahman Bin Faisal University for providing the financial assistance for this study.

Disclosure statement

There is no potential conflict of interest was reported by the author(s).

The study is supported by Imam Abdulrahman Bin Faisal University project application number IRMC-015-2019.