Preparation and exchange transfusion effect of a double polymerization human umbilical cord haemoglobin of red blood cell substitute

Abstract

The development of haemoglobin-based oxygen carrier (HBOC) is an excellent supplement to pre-hospital emergency blood transfusions. In this study, a new type of HBOC was prepared by using human cord haemoglobin (HCHb) and glutaraldehyde (GDA) and Bis(3,5-dibromosalicyl) fumarate (DBBF) to modify (DBBF-GDA-HCHb), the changes of physicochemical indexes during its preparation were evaluated, while a traditional type of GDA-HCHb was prepared, and the oxygen-carrying capacity of two type of HBOC was evaluated by a rat model of 135.0% exchange transfusion (ET). Eighteen SD male rats were selected, and were randomly divided into control group (5.0% albumin), DBBF-GDA-HCHb group and GDA-HCHb group. The 12 h survival rate of the C group was 16.67%, and the two HBOC groups were both 83.33%. Compared with GDA-HCHb, DBBF-GDA-HCHb can reduce lactic acid content by supplying oxygen to hypoxic tissues in a more timely manner, and can also can improve the reduction of MAP due to ischaemia.

Keywords:

• Glutaraldehyde
• Bis(3,5-dibromosalicyl) fumarate
• double polymerization
• red blood cell substitute

Author contributions

Wentao Zhou: Conceptualization, Implementation research and conduction the analysis data, Writing-original draft. Shen Li: Implementation research, Conduction research and Sample preparation. Shasha Hao: Conceptualization, Conduction research, Pharmacological studying in rats and Conduction the analysis. Xintong Xie: Conduction research, Sample detection, Data curation. Honghui Zhang: Conduction research and Sample preparation. Jiaxin Liu: Conceptualization and Supervision. Hong Wang: Conceptualization and Supervision, Interpretation of the data. Chengmin Yang: Conceptualization and Supervision.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

Additional information

Funding

This study was supported by grants from the CAMS Innovation Fund for Medical Sciences (CIFMS), No. 2021-I2M-1-060.