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Abstract
Simple and rapid methods have been developed for the determination of azithromycin, roxithromycin and erythromycin in bulk and pharmaceutical formulations. The methods were based on the formation of bluish-green ion pair complexes of these antibiotics with bromocresol green (BCG) in acetonitrile-ethanol medium. 2:1 complexes were formed between the drug and reagent almost instantaneously with absorption maxima at 630 nm, 620 nm and 625 nm for the three drugs respectively. Under optimum conditions, calibration curves were found to be linear over the range of 4-46 µg mL-1, 3-53 µg mL-1 and 7-73 µg mL-1respectively for the three drugs. The detection limits were found to be 0.19, 0.56 and 0.30 µg mL-1. The methods were subjected to analytical quality control. Recovery studies and interference studies were carried out. The proposed methods were successfully applied to the determination of these drugs in their pharmaceutical formulations and spiked urine samples.