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Abstract
A simple economic approach of RP HPLC-PDA has been used to separate and estimate the tranexamic acid in pharmaceutical formulation. Tranexamic acid being non chromophoric, pre-column derivatization was performed with 2, 4-dinitroflourobenzene solution in aqueous alkaline medium at 60°C. The derivatized drug was then determined using C-18 column (250 × 4.6 mm, 5 μ) and acetonitrile 65% v/v in water as the mobile phase with flow rate of 1 mL/min. The tranexamic acid derivative was detected at a wavelength of 355 nm using PDA detector. Retention time of tranexamic acid was 9.44 min. The method followed Lambert-beers law in the concentration range of 6-16 μg/mL with correlation co-efficient equal to 0.9999. The limit of detection and limit of quantitation were 0.002124 and 0.006434 μg/mL, respectively. The developed method is very sensitive and can be used for determination of tranexamic acid in pharmaceutical formulations.