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Abstract
Quantitative analysis of colorants is of utmost importance in pharmaceutical products as well as in foodstuffs because the elevation of these may lead to the development of health hazards. A reverse phase HPLC method was developed and validated for the estimation of Tartrazine and Brilliant Blue Supra in marketed formulations. The retention time for Tartrazine and Brilliant Blue was 2.847 and 3.512 minutes respectively and this detection was carried out at dual λmax at 427 nm and 629 nm. Linear regression analysis revealed a good linear relationship in the concentration range of 8-12 μg/mL for both Tartrazine and Brilliant blue with R2 values of 0.9966 and 0.9957 respectively. The method showed good reproducibility and recovery with a percentage relative standard deviation (% RSD) < 2 %. The limit for detection (LOD) value was found to be 100 ng for both the dyes whereas the limit of quantitation (LOQ) value was calculated as 302 ng for both the colorants. Photostability studies were carried out under the influence of UV radiations, which revealed approximately 1 % degradation for Tartrazine and 2 % for Brilliant blue after 48 hours.