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Abstract
Hepatoprotective activity of dichloromethane-methanol extracts of Vitex negundo (Linn.) roots was tested against CCl -induced hepatotoxicity in rats in vivo by bioassay guided fractionation. The extract was fractionated by column chromatography and the resulting fractions were analyzed for their hepatoprotective action in rat liver explant cultures in-vitro. The active fraction was further purified by chromatographic method. The 1H & 13C NMR, IR and mass spectral studies of the isolated compound suggested its pentacyclic triterpenic nature, characterized as oleanolic acid acetate, was evaluated for its in vitro hepatoprotective activity. Dichloromethane-methanol extract of Vitex negundo (Linn) root showed a dose dependent in vivo hepatoprotective activity. Treatment of CCl -intoxcicated liver explant culture sets (in vitro) with oleanolic acid acetate at a concentration of 3.3 mg/ml attenuated the marker enzymes (GOT: 27.8±0.73 IU/l; GPT: 17.5±0.09 IU/l and ALP: 16.1±0.66 IU/l) level in culture supernatants significantly (P<0.001) as compared to the toxic. The study revealed significant anti-hepatoxic potential of Vitex negundo (Linn) roots extract and isolated oleanolic acid acetate.