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Abstract
Abstract: The present study was undertaken to investigate the antihepatotoxic activity of ethanolic extract, aqueous extract and compound isolated from the ethanolic extract of the barks of Crataeva nurvala (CN) against CCl induced hepatotoxicity. Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, SALP and bilirubin were tested in vivo in both CCl treated and untreated groups using albino rats. In vitro determination of SGPT and LDH was also carried out using isolated rat hepatocytes. Isolation of compound from the ethanolic extract and its structural elucidation was done simultaneously. From the spectroscopic observations using UV, FTIR, NMR and GCMS, isolated compound was found to be lupeol. Treatment with ethanolic extract (250 and 500 mg/kg p.o.) and isolated lupeol (50 mg/kg p.o.) altered the levels of biochemical markers to almost normal in a dose dependent manner as compared to aqueous extract (250 and 500 mg/kg p.o.). Our findings suggested that CN ethanolic bark extract and the isolated compound (lupeol) possessed antihepatotoxic activity.
Acknowledgements
Authors are grateful to AICTE and ICMR, New Delhi for providing research fellowships to AN and AJ respectively.