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Abstract
The antioxidant activity of three Acacia species such as A. catechu, A. nilotica and A. leucophloea was evaluated using different in vitro techniques like DPPH assay, NO quenching assay, ABTS assay, Metal chelating activity, Hydroxyl radical scavenging activity and Super oxide scavenging activity. Total phenolics, total flavonoids and total tannins were also determined spectrophotometrically. The characterization phenolic compounds were carried out by LC-ESI-MS/MS analysis. Most of the fractions analyzed showed antioxidant activity. The major antioxidant compounds were further identified by tandem mass spectrometry. For the first time, p-coumaric acid was isolated from A. leucophloea and the characterization of the same was done using spectroscopic analysis.