Coronarin-D Production from Tissue Cultured Plantlets of Hedychium coronarium

Abstract

Hedychium coronarium known as white ginger lily, an endangered medicinal plant species belonging to family Zingiberaceae. Coronarin D, a labdane diterpene isolated from rhizomes of H.coronarium inhibits NF-kB activation induced by different inflammatory stimuli and carcinogenesis. In the international market the price of coronarin D is USD 260/5 mg. Callus cultures were initiated, plantlets were regenerated and experimental study of in vitro cultures of H. coronarium was done to estimate the highest presence of coronarin D in lines using different growth regulators as compared to that of the mother plant and its genetic study. Explants were inoculated on Murashige and Skoog media and cultures were maintained. The enhanced coronarin D content from matured rhizomes of established cultures were quantified using High Performance Thin Layer Chromatography with the standard. Inter simple sequence repeats study was performed to characterize the plants genetically. The maximum presence of coronarin D content was found to be 3.51 % whereas in control (mother plant) was 2.35 %. The molecular characterization showed no polymorphism in banding pattern as compared to the control. The present protocol reports somaclone with high coronarin D content which could be further analyzed to be used in large scale for commercial exploitation.

Key words:

• Hedychium coronarium
• improved somaclones
• coronarin D
• HPTLC
• ISSR