Phytobiological Mediated Production of Silver Nanoparticles from Garcinia indica and Their Bactericidal Potential

Abstract

Phytobiological synthesis of nanoparticles is gaining popularity over other conventional methods. The present investigation reports a single - step process to synthesize silver nanoparticles from aqueous extract of Garcinia indica which acts both as a stabilizer and reducing agent. The synthesized nanoparticles exhibited profound activity against human pathogens viz., E. coli (MTCC 7410), S. aureus (MTCC 7443), B. subtilis (MTCC 121) and K. pneumoniae (MTCC 7407) which was confirmed with bactericidal assays. The minimal inhibitory concentration of silver nanoparticles varied from 31.25-12.5 μg/ml against the test pathogens. The synthesized nanoparticles were characterized using UV-visible spectroscopy which revealed the absorption peak at 411 nm. The phyto-constituents bearing functional groups such as OH, C=O, C-N and CH groups responsible for mediating the production of silver nanoparticles was confirmed using Fourier transform infrared spectroscopy (FTIR) analysis. The X- ray diffraction (XRD) studies revealed the crystalline property of synthesized nanoparticles exhibiting Bragg's intensities. Morphological characteristic of synthesized nanoparticles was achieved with Transmission electron microscopy (TEM) which revealed the polydispersity with size ranging from 5 to 50 nm. Among all the test pathogens, Escherichia coli (MTCC 7410) was most sensitive with 12.66 ± 0.57. The study attributes the significant insight on emerging role of silver nanoparticles as potent antimicrobial agents during post antibiotic drug resistant era.

Key words:

• Phytobiological
• Garcinia indica
• Silver nanoparticles
• Bactericidal activity