Expression of MITF Gene in Melanogenesis with Psoralea corylifolia, Zingiber officinale, Terminalia chebula, Punica granatum and Eclipta alba Based on Poly-Herbal Formulation

Abstract

Pigmentation is an important physical characteristic of human beings due to the presence of a biopolymer molecule called melanin. Melanin pigment in the human skin and several other tissues were due to the activation and inhibition of different genes at the downstream signaling pathway like Tyrosinase-related protein 1 (TYRP1) and Tyrosinase-related protein 2 (TYRP2) and Microphthalmia-associated transcription factor (MITF) genes. In this study, we tried to deliver the molecular approach of TYRP1, TYRP2, and MITF by activating kinases in the process of Melanogenesis by treating with Unani formulations on the B16F10 murine melanoma cell line. RNA isolation of B16F10 cells was performed followed by cDNA conversion and expression studies at mRNA levels by RT-PCR method. To explore further western blot was performed at the protein level for the significant results which we obtained with UNIM regimes in the expression studies. The bioactive ingredient of UNIM-004 and UNIM-005 of Methanolic extracts up-regulates the expression and protein levels of the MITF gene which leads to the synthesis of melanin pigment in human beings. Therefore from this study, it indicates that the application and administration of UNIM-004 and UNIM-005 of methanolic extract will result in activating melanin in B16F10 cells by increasing mRNA expression levels in the MITF gene when compared with TYRP1and TYRP2 genes followed by Western blot experiment and found to be producing melanocytes in the progression of Melanogenesis in a dose-dependent manner at 10 μg and 100 μg concerning for to GAPDH control gene at mRNA expression level in Vitiligo patients.

Keywords:

• UNIM regimes
• B16F10 cells
• MITF
• Gene expression
• Western blot
• Skin melanocytes