Effect of crystallization of caffeic acid enhanced stability and dual biological efficacy

Abstract

Caffeic acid (CA) is a well-known natural compound used for both health supplement and metabolic diseases. In the present study, single, easy, and quick isolation procedure of caffeic acid was described from rosmarinic acid. A quick crystal formation also was described for highly hygroscopic CA which was spontaneous and stable with lower hygroscopic nature. In addition, the produced crystal CA was highly stable and showed neuroprotective activity with <6 μg/ml in R28 cell line and cytotoxic activity with >100 μg/ml in U87 MG which revealed its dual biological effect for the treatment of Alzheimer’s disease and cancer, respectively.

Keywords:

• caffeic acid
• rosmarinic acid
• neuroprotective activity
• cytotoxicity
• crystallography
• cancer
• Alzheimer’s diseases

Public Interest Statement

The emerging growing interest to design the formulation in improving drug’s shelf life is becoming viable to eradicate their differential efficacy. In the current study, a kind of viable natural solution of increasing shelf-life of the chemical compound and/or drug was described that showed worth even the smallest risk of differential efficacy in clinical set. The formulation scientist’s genuine interest should look after the drug’s behavior in different states of them under clinical use.

Acknowledgments

We are grateful to the EMAN LAB researcher who helped us during the analysis and experimentation. We are thankful to the USM doctoral fellowship scheme 2015–2016.

Additional information

Notes on contributors

Md Shamsuddin Sultan Khan

The authors are working to find out the differential efficacy of a drug and that solution. This kind of problem is seen during the early development of the drug in clinical case and thereby their failure of approval and marketing. Here, authors are trying to discuss the physical development of a candidate drug which might at least reduce risk of potency of a drug in different clinical cases.