Sweat rate and sweat composition following active or passive heat re-acclimation: A pilot study

ABSTRACT

The purpose of this study was to investigate local sweat rate (LSR) and sweat composition before and after active or passive heat re-acclimation (HRA). Fifteen participants completed four standardized heat stress tests (HST): before and after ten days of controlled hyperthermia (CH) heat acclimation (HA), and before and after five days of HRA. Each HST consisted of 35 min of cycling at 1.5W·kg⁻¹ body mass (33°C and 65% relative humidity), followed by a graded exercise test. For HRA, participants were re-exposed to either CH (CH-CH, n = 6), hot water immersion (water temperature ~40°C for 40 min; CH-HWI, n = 5) or control (CH-CON, n = 4). LSR, sweat sodium, chloride, lactate and potassium concentrations were determined on the arm and back. LSR increased following HA (arm +18%; back +41%, P ≤  0.03) and HRA (CH-CH: arm +31%; back +45%; CH-HWI: arm +65%; back +49%; CH-CON arm +11%; back +11%, P ≤ 0.021). Sweat sodium, chloride and lactate decreased following HA (arm 25–34; back 21–27%, P < 0.001) and HRA (CH-CH: arm 26–54%; back 20–43%; CH-HWI: arm 9–49%; back 13–29%; CH-CON: arm 1–3%, back 2–5%, P < 0.001). LSR increases on both skin sites were larger in CH-CH and CH-HWI than CH-CON (P ≤ 0.010), but CH-CH and CH-HWI were not different (P ≥ 0.148). Sweat sodium and chloride conservation was larger in CH-CH than CH-HWI and CH-CON on the arm and back, whilst CH-HWI and CH-CON were not different (P ≥ 0.265). These results suggest that active HRA leads to similar increases in LSR, but more conservation of sweat sodium and chloride than passive HRA.

Abbreviations: ANOVA: Analysis of variance; ATP: Adenosine triphosphate; BSA (m²): Body surface area; CH: Controlled hyperthermia; CH-CH: Heat re-acclimation by controlled hyperthermia; CH-CON: Control group (no heat re-acclimation); CH-HWI: Heat re-acclimation by hot water immersion; CV (%): Coefficient of variation; dt (min): Duration of a stimulus; F: Female; GEE: Generalized estimating equations; HA: Heat acclimation; HRA : Heat re-acclimation; HST: Heat stress test; LSR (mg·cm⁻²·min⁻¹) : Local sweat rate; LOD (mmol·L⁻¹): Limit of detection; M: Male; $m_x$ (mg): Mass of x; RH (%): Relative humidity; RT: Recreationally trained; SA (cm²): Surface area; t (min): Time; T: Trained; T_sk (°C): Skin temperature; T_re (°C): Rectal temperature; USG : Urine specific gravity; VO_2peak (mL·kg⁻¹·min⁻¹): Peak oxygen uptake; WBSL (L): Whole-body sweat loss; WBSR (L·h⁻¹): Whole-body sweat rate

KEYWORDS:

• Controlled hyperthermia
• heat acclimation
• hot water immersion
• local sweat rate
• sweat composition

Acknowledgments

The authors would like to thank all students and participants who dedicated their time to take part in this study.

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

This work was supported by Eurostars [17213]; ZonMw [2019-30601]; Nederlandse Organisatie voor Wetenschappelijk Onderzoek [P16-28].