Abstract
The complete mitochondrial genome of the Sakhalin nine-spined stickleback Pungitius tymensis was determined using Illumina paired-end sequencing of genomic DNA. The genome sequence was 16 481 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and a control region. The content and arrangement of the genes were identical to those of other Gasterosteidae species. P. tymensis was phylogenetically positioned with other Pungitius species (P. kaibarae, P. pungitius and P. sinensis) with a clear distinction from them. Nucleotide identity in the 37 genic regions ranged from 94.7% to 94.9% between P. tymensis and the other Pungitius species.
Acknowledgements
We thank Pekka Ellonen, Laura Häkkinen, Tiina Hannunen, Kirsi Kähkönen, and Sami Karja for help with laboratory and bioinformatic work. The sequencing was conducted at Finnish Institute for Molecular Medicine
Disclosure statement
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
Funding information
This study was supported by grants (108601 & 118673) from the Academy of Finland.