Characterization of the complete chloroplast genome of Magnolia odoratissima (Magnoliaceae), an endangered and endemic species in China

Abstract

Magnolia odoratissima Law et R. Z. Zhou only produced in Yunnan Province of China. The species with extremely small populations and critically endangered, and now is affirmed as the second most important protection of wild plants in China. In this study, the complete chloroplast genome (cp) of M. odoratissima was sequenced, assembled and analyzed. Our results indicated that the cp genome of M. odoratissima was 159,536 bp in length, containing a large single copy (LSC, 87,688 bp) region and a small single copy (SSC, 18,764 bp) region separated by two reduced inverted repeats (IRs, 26,543 bp). A total of 114 unique genes were identified, including 80 protein-coding genes, 30 transfer RNA (tRNA) genes and 4 ribosomal RNA (rRNA) genes. The overall GC content of M. odoratissima cp genome was 39.3%. Most of the 88 simple sequence repeats (SSRs) were mononucleotides motifs of A/T types and were found to be located in non-coding regions. Phylogenetic analysis of M. odoratissima showed that M. odoratissima was the base group of genus Magnolia with strongly bootstrap supported.

Keywords:

• M. odoratissima
• endangered species
• chloroplast genome
• SSR
• phylogeny

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the National Natural Science Foundation of China under Grant no. 31500459; Science and Technology Plan Projects of Yunnan Province (2016FD024); College fund from College of Horticulture and Landscape, Yunnan Agricultural University (201702), and Horticulture innovative talent training base project ([2015]57).