Characterization of complete mitochondrial genome and gene organization of sharp-spined notothenia, Trematomus pennellii (Perciformes: Nototheniidae)

Abstract

The complete mitochondrial genome of Trematomus pennellii was determined by MiSeq platform. The mitogenome of T. pennellii was 15,976 bp in length and encoded 37 genes (13 proteins, 22 tRNAs, 2 ribosomal RNAs) and a non-coding control region (D-Loop). Overall A + T contents (54.10%) in the mitogenome was higher than G + C contents (45.90%). Twenty-nine genes were located on H strand, whereas eight genes were identified on L strand. Among 13 protein-coding genes, unusual start codons were identified in COX1 (GTG), ND6 (ATT), COX2 (ATC), and ATP6 (ATC). The incomplete stop codon (T–) was identified in six genes including COXII, COXIII, NAD2, NAD3, NAD4, and Cytb. The phylogenetic tree of complete genome indicated that Trematomus pennellii was most closely related to Trematomus bernacchii with 91.65% DNA sequence identity.

Keywords:

• Next generation sequencing
• Trematomus pennellii
• mitochondrial genome
• Antarctica
• Notothenioidei

Disclosure statement

The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Additional information

Funding

This research was supported by the ‘Ecosystem Structure and Function of Marine Protected Area (MPA) in Antarctic project [PM1818060]’, funded by the Ministry of Oceans and Fisheries [20170336], Korea.