Abstract
The complete mitochondrial DNA genome of Tatia intermedia was first reported using next-generation sequencing method. The entire length of mitochondrial genome is 16,582 bp and the nucleotide composition was made up of 31.4% A, 26.7% T, 26.2% C, and 15.7% G, respectively, indicating an A + T (58.1%)-rich feature. With the exception of eight tRNA genes and NADH6, mitochondrial genes are encoded on the heavy strand, which was similar to that in other vertebrates. The phylogenetic relationship of T. intermedia and other Auchenipteridae species showed that the species of Auchenipteridae were gathered in the same branch.
The Auchenipteridae are Neotropical catfish belongs to Siluriformes, which are distributed from Panama to Argentina (Royero, Citation1999). There are two subfamilies, 24 genera and 121 valid species in this family, but only three complete mtDNA sequences are available (Sato et al. Citation2018). Specimens of Tatia intermedia were collected from Huadiwan Flowers and Birds market in Guangzhou, China and kept in the Fisheries Research Institute, Academy of Agricultural Sciences of Guizhou, Guiyang, China. In the present study, the complete mitochondrial DNA genome of Tatia intermedia was first reported using next-generation sequencing method. The complete mitochondrial sequence of T. intermedia had been deposited in the GenBank with an accession number MK078120. The entire length of mitochondrial genome is 16,582 bp and the nucleotide composition was made up of 31.4% A, 26.7% T, 26.2% C, and 15.7% G, respectively, indicating an A + T (58.1%)-rich feature. With the exception of 8 tRNA genes and NADH6, mitochondrial genes are encoded on the heavy strand, which was similar to that in other vertebrates (Yue et al. Citation2006; Ma et al. Citation2014). Except for overlapped between NADH5 and NADH6 encoded on opposite strands, some overlaps were also appeared on 13 protein-coding genes encoded on the same strand. The similar phenomena have already shown in most vertebrates (e.g., Gu et al. Citation2007; Vittas et al. Citation2011).
The phylogenetic relationship of T. intermedia and other Auchenipteridae species was inferred using the neighbor-joining and maximum likelihood method in MEGA 7.0 (Kumar et al. Citation2016). The results showed that the species of Auchenipteridae were gathered in the same branch. T. intermedia and Centromochlus perugiae were sister group (). this report could enrich the mitogenome resource of T. intermedia and it also seems to be useful for evolutionary and conservation studies on Auchenipteridae and Siluriformes fish species
Figure 1. Neighbor-joining phylogenetic tree of the T. intermedia and other species based on the complete mitochondrial genome. Numbers on nodes indicate bootstrap support value, based on 1000 replicates.
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References
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