Abstract
Hemitripterus villosus is an important economic fish. With the completion of H. villosus mitochondrial genome sequencing, the full-length mitochondrial genome of H. villosus was assembled and analyzed. All results indicate that the complete mitochondrial genome of H. villosus was 17,228 bp. There were 22 tRNAs and 13 protein-coding genes (PCGs), and 2 rRNA. Besides, the phylogenetic tree of 19 teleost fish was constructed. The results showed that H. villosus was clustered with other sculpins, which including Cottus reinii, C. czerskii and Hexagrammos otakii. This mitogenome will contribute to the further systematics and evolution study of H. villosus.
Hemitripterus villosus, an important economic fish that lived in coastal waters. It belongs to the phyla Actinopterygii, order Scorpaeniformes, family Cottidae, genus Hemitripterus, mainly distributed in the Northwest Pacific coast, also distributed in the North of China and Japan (Munehara Citation1992). Due to the overfishing of H. villosus and the infection of Anisakis in recent years (Du Citation2006), its natural production has severely affected. To provide an efficient tool to study the biodiversity and conversation of this fish, we sequenced its mitogenome.
In the current study, H. villosus was collected in the coast of Yellow sea in Dalian, Liaoning province, North China (121.5591 E, 38.8681 N). The genomic DNA was extracted from the muscle. The specimen was stored at Dalian Ocean University (specimen code DOU190405). With the completion of H. villosus mitochondrial genome sequencing by HuiTong Biological Technology Co., Ltd (Shenzhen, China) via Illumina MiSeq Next-generation sequencing technique, the full-length mitochondrial genome of H. villosus was assembled with CAP3 (Huang and Madan Citation1999) and submitted to the GenBank database (GenBank Accession: MN316630). All analytical results indicate that the complete mitochondrial genome of H. villosus was 17,228 bp (). There were 22 tRNAs (trnF-GAA, trnV-TAC, trnL-TAA, trnI-GAT, trnQ-TTG, trnM-CAT, trnW-TCA, trnA-TGC, trnN-GTT, trnC-GCA, trnY-GTA, trnS-TGA, trnD-GTC, trnK-TTT, trnG-TCC, trnR-TCG, trnH-GTG, trnS-GCT, trnL-TAG, trnE-TTC, trnT-TGT, trnP-TGG), 13 protein-coding genes (PCGs), and 2 rRNA. Among these PCGs, twelve genes (nd1, nd2, cox2, atp8, atp6, cox3, nd3, nd4l, nd4, nd5, nd6, cytb) were with start codon ATG, while the cox1 was with start codon GTG. Besides, eight PCGs use TAA as the stop codon and three PCGs (nd3, atp8, and atp6) use TAG as the stop codon. Two PCGs (nd2 and nd4) use AGG as the stop codon ().
Figure 1. Phylogeny of 19 teleost fish based on amino acid sequences of PCGs located in the mitogenome. The number of the branches denoted NJ posterior probabilities.
Based on sequences of 13 PCGs (nd1, nd2, cox1, cox2, atp8, atp6, cox3, nd3, nd4l, nd4, nd5, nd6, cytb), the phylogenetic tree of 19 teleost fish was constructed by maximum likelihood method. The phylogenetic tree () showed two main clusters: Choanichthyes and Actinopterygii. The H. villosus was clustered with other sculpins, including Cottus reinii, cottus czerskii and Hexagrammos otakii. The relationships displayed in the phylogenetic tree corresponded with their taxonomic classification.
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No potential conflict of interest was reported by the author(s).
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References
- Du JY. 2006. Ecological features and stock enhancement prospects of Hemitripterus villosus. Beijing Fisheries. 5:22–25.
- Huang X, Madan A. 1999. CAP3: a DNA sequence assembly program. Genome Res. 9(9):868–877.
- Munehara H. 1992. Utilization of polychaete tubes as spawning substrate by the sea raven Hemitripterus villosus (Scorpaeniformes). Environ Biol Fish. 33(4):395–398.