Abstract
The complete mitochondrial (mt) genome of Nassarius gregarius was determined using genome walking techniques in this study. The total length of the mt genome sequence of N. gregarius was 15,169 bp, including 13 protein-coding genes, 23 transfer RNA genes, and 2 ribosomal RNA genes. The overall composition of the mitogenome was estimated to be 30.46% for A, 39.54%% for T, 14.38% for C, and 15.52% for G, indicating that an A + T (70.00%)-rich feature occurs in the N. gregarius mitogenome. The phylogenetic relationships of 13 mollusk species were constructed based on the complete mtDNA sequences by the neighbor-joining method using MEGA 7.0 and DNAMAN 6.0 software.
The Nassarius gregarius belongs to the family Nassariidae and could be found in northern parts Yellow Sea and Bohai Sea of China. The population of wild N. gregarius is not very small, but the research about N. gregarius is rare. In order to provide some biological data for N. gregarius, it is necessary to carry out some wild resource investigation and germplasm analysis. In this study, we report the complete sequence of mitochondrial genome for N. gregarius (GenBank accession no. MT075611). The findings will provide useful information for further studies on population genetics, phylogenetic construction and other relevant studies in N. gregarius.
One N. gregarius individual was collected from Dalian Sea area, Liaoning Province, China (39°11′23″N, 124°47′24″E). The total genomic DNA was extracted from foot muscle by a modification of standard phenolchloroform procedure. The complete mitogenome of N. gregarius was sequenced by primer walking. The gene annotation was performed following the methods described by Hao et al. (Citation2016) and Online software (GeneMarker, tRNAscan, and ORFfinder). At present, the N. gregarius specimens were stored in the Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, P.R. China, Dalian Ocean University, Dalian, 116023, Liaoning, China. The specimens were numbered DLOU -KLM-SSH06 to DLOU-KLM-SSH09.
The total length of the hybrid of N. gregarius mitochondrial genome was 15,169 bp, with the base composition of 30.46% A, 39.54% T, 14.38% C, and 15.52% G. It comprised 2 ribosomal RNA genes, 13 protein-coding genes, and 23 transfer RNA genes. All the mitogenome genes were encoded on the heavy strand except for 11 tRNA genes (tRNA-Asp, tRNA-Glu, tRNA-Gly, tRNA-Ala, tRNA-Arg, tRNA-Asn, tRNA-Ser, tRNA-Ile, tRNA-(Lys/Asn), and tRNA-Thr).
The phylogenetic analysis showed that the complete mitochondrial sequence of N. gregarius was phylogenetically closer to Tritia reticulatus by 82.84% bootstrap support and formed the Nassariidae clade (). Our result was consistent with the previous researches, both in traditional morphological and molecular-based phylogeny studies. We expect that the present result can contribute to construct molecular identification of this species and be helpful to explore the phylogeny of Nassariidae.
Figure 1. Consensus neighbor-joining tree based on the complete mitochondrial sequence of N. gregarius and other 12 mollusk species. The phylogenetic tree was constructed using MEGA 7.0 and DNAMAN 6.0 software by the neighbor-joining method. The numbers at the tree nodes indicates the percentage of bootstrapping after 1000 replicates.
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Reference
- Hao Z, Yang L, Zhan Y, Tian Y, Wang L, Mao J, Chang Y. 2016. The complete mitochondrial genome of Neptunea arthritica cumingii Crosse, (Gastropoda: Buccinidae). Mitochondrial DNA Part B. 1(1):220–221.