Complete mitochondrial genome of Gymnodiptychus pachycheilus weiheensis (Teleostei: Cypriniformes: Cyprinidae)

Abstract

This study provides a mitochondrial complete genome of Gymnodiptychus pachycheilus weiheensis. The complete genome is 16,680 bp in length with an A + T content of 55.5%, contains 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. The phylogenetic analysis indicated that Gymnodiptychus pachycheilus weiheensis is closed related to Oxygymnocypris stewartii. These results contribute to explore the molecular evolution of Gymnodiptychus pachycheilus weiheensis.

Keywords:

• Gymnodiptychus pachycheilus weiheensis
• mitochondrial genome
• phylogenetic analysis

Gymnodiptychus pachycheilus weiheensis belongs to the genus Gymnodiptychus, in the subfamily of Schizothoracinae (Cypriniformes, Cyprinidae), is distributed in the upper reaches of the Weihe River, and the spot at altitude of 1360 m to 1600 m (Song and Wang 1984; Wang and Song 1985; Zhang et al. 2013). Gymnodiptychus pachycheilus weiheensis is the endemic cold-water species in China’s western Gansu province, with the high economic value (Song and Wang 1984; Wang and Song 1985). There are some big differences of geographical distribution, morphological characteristics and genetic divergence between Gymnodiptychus pachycheilus weiheensis and Gymnodiptychu pachycheilus, which were specialized-grade Schizothoracinae species (Zhang et al. 2013). Gymnodiptychus pachycheilus weiheensis is the omnivore, feeding on aquatic insects, shrimp, zooplankton, fragments of plant and algae (Zhang et al. 2013). Compared to Gymnodiptychu pachycheilus, Gymnodiptychus pachycheilus weiheensis has sparsely outside and inside gill-raker on first gill arch, no spots on dorsal fin and tail fin, and the low depth of caudal peduncle in length of caudal peduncle on the morphological characteristics (Zhang et al. 2013). In recent years, some researches focused on the biological characteristics and captive breeding, but there was lack of the genomic information of the Gymnodiptychus pachycheilus weiheensis. Therefore, in this study we established a complete mitochondrial genome of Gymnodiptychus pachycheilus weiheensis to explore the inheritance and evolution.

In this study, Gymnodiptychus pachycheilus weiheensis were collected from the Wushan County (Gansu, China; 104°92′E, 34°72′N) and voucher specimens (Wushan-001) were deposited at the Museum of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University. The sample of Gymnodiptychus pachycheilus weiheensis was frozen in liquid nitrogen immediately after collection and stored at –80 °C. The complete mitogenome sequence of Gymnodiptychus pachycheilus weiheensis was deposited into GenBank dayabase with accession number (MT192766). The whole length of the mitogenome of Gymnodiptychus pachycheilus weiheensis is 16,680 bp, with an A + T content of 55.5%. The whole mitogenome contains 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes.

We constructed a phylogenetic tree (Figure 1) by using RAxML 8.1.17 (Stamatakis 2014). The results demonstrated that Gymnodiptychus pachycheilus weiheensis was closely related with Oxygymnocypris stewartii (Figure 1).

Figure 1. The maximum likelihood tree of the Gymnodiptychus pachycheilus weiheensis and 10 other species with Genbank accession numbers.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Natural Science Foundation of Qinghai Province of China [2020-ZJ-944Q], and the National Natural Science Foundation of China [31960127], and the Scientific research training project of the College of Agriculture and Animal Husbandry of Qinghai University. Dr. Shuo Feng and Zhifang An were supported by ‘1000 Talent’ programs of Qinghai Province.