Complete mitochondrial genome of the gray reef shark, Carcharhinus amblyrhynchos (Carcharhiniformes: Carcharhinidae)

Abstract

We report the first mitochondrial genome sequences for the gray reef shark, Carcharhinus amblyrhynchos. Two specimens from the British Indian Ocean Territory were sequenced independently using two different next generation sequencing methods, namely short read sequencing on the Illumina HiSeq and long read sequencing on the Oxford Nanopore Technologies’ MinION sequencer. The two sequences are 99.9% identical and are 16,705 base pairs (bp) and 16,706 bp in length. The mitogenome contains 22 tRNA genes, two rRNA genes, 13 protein-coding genes and two non-coding regions; the control region and the origin of light-strand replication (OL).

Keywords:

• Shark
• British Indian Ocean Territory
• Carcharhinus amblyrhynchos
• mitochondrial genome

Disclosure statement

No potential conflict of interest was reported by the author(s).

Author contributions

ND and SJ contributed to concept, bioinformatics and analysis, ND wrote the manuscript with comments from all authors, SJ contributed to sequencing and EAD provided laboratory support. BAB, VS and DC contributed to the concept of the manuscript and financial support of the project. BAB, TKC and DC contributed the samples.

Data availability statement

The data that support the findings of this study are openly available in GenBank at https://www.ncbi.nlm.nih.gov/nuccore/MT093205 reference number MT093205.1 and https://www.ncbi.nlm.nih.gov/nuccore/MT104515 reference number MT104515.1.

Additional information

Funding

We thank the Bertarelli Foundation and the UK Natural Environment Research Council for funding, Robert Edwards and Adrian Cantu for computing support, Danny Coffey, Tim White, Nathan Truelove, Robert Schallert, and David Jacoby for collecting samples, Theodore Reimer for logistics and CD-genomics for assistance with Illumina sequencing.