Characterization of the complete chloroplast genome of Alsophila spinulosa, an endangered species endemic to China

Abstract

Tree fern Alsophila spinulosa is an endangered relic plant in the world. It is currently on the International Union for Conservation of Nature (IUCN) red list of threatened species. In this study, we first assembled the complete chloroplast (cp) genome of A. spinulosa by Illumina paired-end reads data. The whole genome was 156,661 bp, consisting of a pair of inverted repeats of 24,364 bp, large single copy region and a small single copy region (70,352 and 21,624 bp in length, respectively). The cp genome contained 133 genes, including 92 protein-coding genes, 33 trRNA genes, and eight rRNA genes. The overall GC content of the whole genome was 40.4%. A neighbour-joining phylogenetic analysis demonstrated a close relationship between A. spinulosa and Cystoathyrium chinense Ching.

Keywords:

• Alsophila spinulosa
• endangered species
• chloroplast genome

Acknowledgements

Thanks to Gao, L., Yi, X., Yang, Y.X., Su, Y.J. and Wang, T. for the research provided a theoretical basis for my previous data. (Complete chloroplast genome sequence of a tree fern Alsophila spinulosa: insights into evolutionary changes in fern chloroplast genomes.)

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statements

The data that support the findings of this study are openly available in figshare at 10.6084/m9.figshare.12250268.

Additional information

Funding

The research was awarded the Jilin Provincial Natural Science Foundation Project [20190201168JC], Jilin Province Education Department Science and Technology Research Project [JJKH20180661KJ], Jilin Province Key Technology R&D Project [20180201029NY], Jilin Province. Support for the Science and Technology Development Program [20190802012ZG] and Jilin Province Science and Technology Guidance Project [20180312006ZG].