Sequence analysis of mitochondrial genome of the false and phantom crane-fly Ptychoptera qinggouensis Kang, Yao and Yang, 2013 (Diptera, Ptychopteridae)

Abstract

The genus Ptychoptera Meigen, 1803 is the largest genus of the family Ptychopteridae with 78 known species. In this study, we report a nearly complete mitochondrial (mt) genome of this genus, which is a circular molecule of more than 15,028 bp. The mt genome contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a non-coding region. The overall base composition is A (38.1%), T (36.7%), C (14.9%), and G (10.4%), with an AT content of 74.8%. The AT content of N-strand genes (75.7%) is higher than that of the J-strand genes (71.7%). Phylogenetic analysis reveals that the monophyly of Ptychopteridae, Bibiomorpha, Tipulomorpha and Brachycera are strongly supported, and the sister group relationship between Tanyderidae and Ptychopteridae is not supported.

Keywords:

• Mitochondrial genome
• phylogeny
• Ptychoptera
• false and phantom crane-fly

Acknowledgements

We express our sincere thanks to Ding Yang (Beijing) and Fan Song (Beijing) for great help during the study. We are also very grateful to Xin Li (Beijing) for collecting the specimen.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study are openly available in NCBI at https://www.ncbi.nlm.nih.gov/. GeneBank accession numbers are listed as follows: Aedes albopitus (NC_006817), Anopheles darlingi (NC_014275), Arachnocampa flava (NC_016204), Atylotus miser (KT225291), Bittacomorphella fenderiana (JN_861745), Bittacus pilicornis (NC_015118), Boreus elegans (NC_015119), Chaoborus sp. (MK281356), Chironomus tepperi (NC_016167), Chrysops silvifacies (KT225292), Corethrella condita (MK281357), Cramptonomyia spenceri (NC_016203), Culex quinquefasciatus (NC_014574), Cydistomyia duplonotata (NC_008756), Cylindrotoma sp. (KT970060), Dialysis sp. (KT225293), Dixella aestivalis (NC_029354.1), Dixella sp. (KM245574), Dolichopus bigeniculatus (KT225294), Dorcadia ioffi (NC_036066.1), Drosophila yakuba (NC_001322), Haemagogus janthinomys (NC_028025.1), Haematobia irritans (NC_007102), Heleodromia immaculata (KT225295), Jellisonia amadoi (NC_022710.1), Leptogaster longicauda (KT225296), Limonia phragmitidis (NC_044484.1), Liriomyza bryoniae (NC_016713), Neopanorpa pulchra (NC_013180.1), Nyssomyia umbratilis (NC_026898), Ochlerotatus vigilax (NC_027494), Paracladura trichoptera (NC_016173), Parochlus steinenii (KT003702), Pedicia sp. (KT970062), Phlebotomus chinensis (KR349297.1), Phlebotomus papatasi (KR349298.1), Polypedilum vanderplanki (NC_028015.1), Protoplasa fitchii (NC_016202), Ptecticus aurifer (KT225297), Ptychoptera qinggouensis (MT380468), Ptychoptera sp. (NC_016201), Rhagio sp. (KT225298), Rhamphomyia insignis (KT225299), Rhipidia chenwenyoungi (KT970063), Satanas sp. (KT225300), Simosyrphus grandicornis (NC_008754), Simulium aureohirtum (KP793690.1), Simulium quinquestriatum (MK281358), Suragina sp. (KT225301), Thaumalea sp. (MK281359), Tipula abdominalis (JN_861743), Tipula cockerelliana (KT970065), Tipula nova (MN583330), Trichocera bimacula (NC_016169), Trichophthalma punctata (NC_008755) and Xylomya moiwana (KT225302).

Additional information

Funding

This work was funded by the National Natural Science Foundation of China [41901061], the Shandong Provincial Natural Science Foundation, China [ZR2019BC034] and the High-level Talents Funds of Qingdao Agricultural University, China [663-1119008].