Characterization of the first chloroplast genome of Tabebuia (Bignoniaceae)

Abstract

The chloroplast genome of Tabebuia nodosa is described and characterized here. This species is endemic to the Chaco and the first species of Tabebuia to have its organelle genome sequenced, providing a genomic resource for phylogenetic inferences. The plastome of T. nodosa is 158,454 bp in length, with a large single-copy of 85,406 bp, a small single-copy of 12,785 bp, and inverted repeats of 30,116 bp each. It contains 131 genes, with 86 protein-coding genes, 37 tRNA, and 8 rRNA. Overall, the GC content is 38.2%. The T. nodosa plastome resembles the structural organization of plastomes commonly found in flowering plants, including those of other genera of Bignoniaceae. A phylogenetic analysis combining a subset of Bignoniaceae plastomes confirms the placement of T. nodosa within the Tabebuia alliance with maximum support.

Keywords:

• Bignoniaceae
• chloroplast genome
• phylogeny
• Chaco

Acknowledgments

We thank the Core Facility for Scientific Research from the Universidade de São Paulo (CEFAP-USP/GENIAL) for allowing us to use the Covaris S2 sonicator, Qubit, and the SEAL server.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study are available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov, reference number MT447061.

Additional information

Funding

L.H.M.F. is supported by a CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) postdoctoral fellowship, and L.G.L. is supported by a Pq-1B grant [310871/2017-4] from CNPq [Conselho Nacional de Desenvolvimento Científico e Tecnológico].