The complete mitochondrial genome of the Picasso clownfish: genomic comparisons and phylogenetic inference among Amphiprioninae

Abstract

Picasso clownfish belong to the subfamily Amphiprioninae and are considered a variant of the genus Amphiprion. In this study, we first sequenced the complete mitochondrial genome of the Picasso clownfish by Illumina next-generation sequencing technology. The length of the whole mitogenome is 16,727 bp long, with a gene arrangement and composition similar to those of two other Amphiprion species (Amphiprion ocellaris and Amphiprion percula). The topological structure of the phylogenetic tree shows that the Picasso clownfish is more closelyrelated to A. percula than it is to A. ocellaris, suggesting that the Picasso clownfish may be a variant of A. percula.

Keywords:

• Picasso clownfish
• next-generation sequencing
• mitochondrial genome
• phylogenetic relationships
• comparative analysis

Disclosure statement

No potential conflict of interest was reported by the authors.

Authors’s contributions

HE Li-bin conceived and designed the study; HE Li-bin and WU Shui-qing collected the data; HE Li-bin and LUO Hui-yu performed the RNA-Seq analysis. HE Li-bin and ZHENG Le-yun wrote the manuscript. All authors read, edited, and approved the final manuscript for submission.

Data availability statement

The data that support the findings of this study are openly available in GenBank database at https://submit.ncbi.nlm.nih.gov/subs/sra/SUB3297187/overview, reference number [SRR6363367].

Additional information

Funding

This work was supported by the Special Fund for Marine and Fishery Structural Adjustement from Finance Department of Fujian Province [No.841[2016] and [2019HYJG11]) and the Science and Technology Plan Special Funding of Xiamen Science and Technology Bureau [3502Z20142008].