Abstract
Dendrobium wangliangii is a rare orchid species with extremely small populations, endemic to China. In this study, the complete chloroplast (cp) genome sequence and the genome features of D. wangliangii were analyzed. The whole cp genome sequence of D. wangliangii is 160,052 including a large single-copy region (LSC, 87,525 bp), a small single-copy region (SSC, 18,373 bp), and a pair of repeat regions (IRs, 27,077 bp, each). The contents of four bases in cpDNA were A (30.9%), C (18.9%), G (18.3%) and T (31.9%), respectively. The total content of GC is 37.1%. The cp genome contains 129 genes, consisting of 124 unique genes (78 protein-coding genes, 38 tRNAs, and 8 rRNAs). Phylogenetic analysis showed that D. wangliangii nested with other Dendrobium spp. and was closely related to D. ellipsophyllum, D. wattii and D. longicornu.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The data that support the analyses and results of Dendrobium wangliangii in this paper are available in GenBank with accession no. MT798588 (https://www.ncbi.nlm.nih.gov/). Raw sequencing reads was deposited in SRA with BioProject accession: PRJNA659161. (https://www.ncbi.nlm.nih.gov/Traces/study/?acc=PRJNA659161).
Acknowledgments
The authors thank Dr. Wen-Bin Yu for help in software applications and Ms. Bi-Ying Yue for lab assistance during DNA extraction. We are also grateful to Mrs. Xiao-Yun Wang from the Wild Orchid conservation Center of Yunnan Fengchunfang Biotechnology Company for providing sample materials.