Characterization and phylogenetic analysis of the complete mitochondrial genome of Pseudothemis zonata (Odonata: Anisoptera: Libellulidae)

Abstract

Pseudothemis zonata is a commonly seen dragonfly with a big yellow or white ringlike spot on the third and fourth segments of its abdomen. In this study, we sequenced and analyzed the complete mitochondrial genome (mitogenome) of P. zonata. This mitogenome was 15,434 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and 2 ribosomal RNA unit genes (rRNAs). Gene order was conserved and identical to most other previously sequenced Libellulidae dragonflies. The whole mitogenome exhibited heavy AT nucleotide bias (74.6%). Most PCGs of P. zonata have the conventional start codons ATN (six ATG, three ATT, and two ATC), with the exception of cox1 and nad1 (TTG). Except for four genes (cox1, cox2, cox3, and nad5) end with the incomplete stop codon T−, all other PCGs terminated with the stop codon TAA or TAG. Phylogenetic analysis showed that P. zonata got together with Brachythemis contaminata with high support value, and the relationships ((Brachythemis + Psolodesmus) + ((Hydrobasileus + Trigomphus) + (Orthetrum + Acisoma))) were supported in Libellulidae.

Keywords:

• Libellulidae
• mitochondrial genome
• Pseudothemis zonata
• phylogenetic analysis

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study are openly available in NCBI (National Center for Biotechnology Information) at https://www.ncbi.nlm.nih.gov/, reference number MT371043.

Additional information

Funding

This study was supported by the Foundation of Jiangxi Province Educational Committee [GJJ190759] and Gannan Normal University Bidding Project in 2016 [480070].