Comparative analysis of mitochondrial genomes in genetically distinct groups of the dojo loach Misgurnus anguillicaudatus

Abstract

Dojo loach (Misgurnus anguillicaudatus) that inhabit Japan are composed of two genetically divergent groups (A and B). Although most individual loach reproduce bisexually, clone lineages exist in certain populations that reproduce gynogenetically. To investigate the molecular phylogenetic relationships among the M. anguillicaudatus groups and clone lineages, complete mitogenomes of members from groups A and B and a clone lineage were sequenced using long range PCR and primer walking methods. The three groups of mitogenomes shared the same gene order and had similar base compositions and codon usage patterns. Phylogenetic analysis indicated group A and the clone lineage were genetically close with group B being genetically divergent.

Keywords:

• Dojo loach
• Misgurnus anguillicaudatus
• mitochondrial genome

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study are available in ‘DNA Date Bank of Japan’ at http://getentry.ddbj.nig.ac.jp/, accession number [LC532166, LC532167, LC532168].

Acknowledgments

We thank Dr. Fumihito Tashiro, (Hokkaido University Museum, Fisheries Science Center) for assistance with archival of the M. anguillicaudatus specimen DNAs at HUMZ.

Additional information

Funding

Duong Thuy Yen participated in this work during her visit to the Laboratory of Aquaculture Genetics and Genomics under the Can Tho University Improvement Project VN14-P6, supported by a Japanese Official Development Assistance (ODA) loan.