Abstract
The mudskipper, Boleophthalmus pectinirostris (B. pectinirostris), is an amphibious fish that lives in the intertidal mudflats. It is a cultured economic fish with nutritional and pharmacological value. Here, we report the complete mitochondrial genome sequence of B. pectinirostris, which is 17,111 base pairs (55.3% A + T content) in length and consists of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and a 1453 bp D-loop region. The complete mitochondrial genome of B. pectinirostris will provide useful genetic information for future phylogenetic and taxonomic classification of B. pectinirostris.
The mudskipper, Boleophthalmus pectinirostris (Gobiiformes, Oxudercidae) is an amphibious gobioid teleosts, which is a commercially important aquaculture species (Zhao et al. Citation2018). It is a warm water fish that distributes in tropical mudflats along the coast of Japan, Taiwan and Southern China (Clayton Citation1993; Liu et al. Citation2012). B. pectinirostrislives in the intertidal zone along the coastland estuarine areas where the salinity change is intense, hence it shows salinity tolerance (Chen et al. Citation2006). The physiological, biochemical and adaptive mechanisms of B. pectinirostris have become hot research issues, due to its habitat specificity and unique amphibious adaptability. Natural populations of B. pectinirostris are gradually decreasing due to the overfishing and habitat destruction (Zhang and Hong Citation2006). Mitochondrial DNA (mtDNA) is particularly suitable for evolutionary studies because it has the unique characteristics of strict maternal inheritance, high copy number, and rapid evolution (Stoneking and Soodyall Citation1996). The complete mitochondrial genome is an excellent molecular marker for studying phylogenetic relationships and species identification (Zhong et al. Citation2020). Here, we report the complete mitochondrial genome of B. pectinirostris and the analyses of the mitogenomic phylogenetic. The results of this study will provide reference for genetics research of B. pectinirostris.
The B. pectinirostris samples were collected from Hepu (21.593357 N, 109.087536E), Beihai, Guangxi, China. The whole body specimens (#BH201905310001) were deposited at Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, China. The total genomic DNA was extracted from the fins of one B. pectinirostris samplevia the phenolchloroform extraction method (Kumar and Mugunthan Citation2018). DNA libraries (350 bp insert) were constructed using the TruSeq NanoTM kit (Illumina, San Diego, CA) and were sequenced (2 × 150bp paired-end) on a HiSeq platform by Novogene Company, China. Sequence assembly was performed using the MITObim software (Hahn et al. Citation2013). Gene annotation was performed using the MITOS software (http://mitos2.bioinf.uni-leipzig.de/). The phylogenetic tree was constructed using maximum-likelihood method.
The complete mitochondrial genome of B. pectinirostris is 17,111 bp in length (GenBank accession number: MN909967) with the base composition of A (25.7%), T (29.6%), C (29.0%) and G (15.7%). The percentage of G + C is 44.7%, and the percentage of A + T is 55.3%, which is similar to the giant mudskipper, Periophthalmodon schlosseri (42.4% of G + C and 57.6% of A + T) (Yi et al. Citation2016). The mitochondrial genome of B. pectinirostris contains 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs (a 12S rRNA and a 16S rRNA), and a D-loop region.
The mitogenomic phylogenetic analyses showed that B. pectinirostris was first clustered with B. pectinirostris collected from Cixi, China (NC 016195.1), whose complete mitochondrial genome is 17,111 bp in length, base composition is 29.7% for A, 28.3% for C, 26.6% for T and 15.4% for G, which are similar to those of the B. pectinirostris in this study, but the content of A + T (56.3%) is higher (55.3% in this study). Then, B. pectinirostris was clustered with Boleophthalmus boddarti (B. boddarti) (), indicating the close relationship between B. pectinirostris and B. boddartiThe complete mitochondrial genome sequence of B. pectinirostris will enrich the genome data of Gobiiformes, and will be useful for taxonomy research, conservation, and management.
Figure 1. A phylogenetic tree was constructed based on the comparison of mitochondrial genome sequences of B. pectinirostris and other species of Gobiidae. All the sequences were downloaded from NCBI GenBank.
Disclosure statement
The authors declare that they have no conflict of interest.
Data availability statement
The data that support the findings of this study are openly available in National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov) under the accession MN909967. The associated BioProject, SRA, and BioSample numbers are PRJNA688752, SRR13334530, and SAMN17188496822, respectively.
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References
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