Complete mitochondrial genomes of two marine monogonont rotifer Brachionus manjavacas strains

Abstract

The complete mitochondrial genomes of Brachionus manjavacas German strain were 10,721 bp (mitochondrial DNA I) and 12,274 bp (mitochondrial DNA II) in size, while the complete mitochondrial genomes of B. manjavacas Australian strain were 10,889 bp (mitochondrial DNA I) and 12,443 bp (mitochondrial DNA II) in size. Of 12 protein-coding genes (PCGs), 99.6% of amino acid sequences were identical between the two strains. Of 12 PCGs of both B. manjavacas strains, three genes (ND1, ATP6, and ND5) had incomplete stop codon T. Furthermore, ATA was the start codon for ND4, ND5, and CO3 genes, whereas that for other PCGs was ATG in both strains. The base compositions of 12 PCGs in the B. manjavacas strains were similar, indicating that the mitochondrial genome of the two strains was structurally conserved over evolution. The gene structure and its orientation of 12 PGCs of B. manjavacas strains were identical, as shown in other marine Brachionus rotifers and the freshwater Brachionus rotifers, while the freshwater rotifer B. calyciflorus had an additional cytochrome b gene in the mitochondrial DNA I.

Keywords:

• Monogonont rotifer
• complete mitochondrial genome
• Brachionus manjavacas
• Australian strain
• German strain

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Data availability statement

The genome sequence data that support the findings of this study for B. manjavacas Australian strain are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/nuccore under the accession no. MW559986-MW559987. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA716452, SRR14040032, and SAMN18436264, respectively. The genome sequence data that support the findings of this study for B. manjavacas German strain are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/nuccore under the accession no. MW559988-MW559989. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA716453, SRR14040033, and SAMN18436265, respectively.

Additional information

Funding

This research was supported by the Collaborative Genome Program of the Korea Institute of Marine Science and Technology Promotion funded by the Ministry of Oceans and Fisheries [No. 20180430] and also supported by a grant from the National Institute of Fisheries Science, Korea [R2021033].