Characterization of the complete mitochondrial genome of Haemadipsa tianmushana Song 1977 (Hirudiniformes, Haemadipsidae) and its phylogenetic analysis

Abstract

The complete mitochondrial genome of Haemadipsa tianmushana Song 1977 from China has been determined and reported for the first time in this study. It was 14,625 bp in length and consisted of 22 tRNA genes, 2 rRNA genes, 13 protein-coding genes (PCGs), and 3 control regions. The nucleotide base content of the complete mitogenome for this species was 35.1% A, 10.5% C, 11.6% G, and 42.8% T. The tRNA genes were ranged from 57 bp (Ser^TCT) to 66 bp (Gln^TTG) in length. The phylogenetic analyses indicated that Hirudinea is a mono-phyletic clade. And it includes Whitmania acranulata, Whitmania pigra, Whitmania laevis, Zeylanicobdella arugamensis, Ozobranchus jantseanus and Placobdella lamothei. In Hirudiniformes, H. tianmushana and three species of Haemopidae were obviously clustered into two independent branches. This result is consistent with a taxonomy that they all belong to the same suborder. This study adds to the genetic resources currently available for the species.

Keywords:

• Blood-feeding leeches
• complete mitochondrial genome
• Haemadipsa tianmushana
• phylogeny
• Haemadipsidae

Disclosure statement

No potential conflict of interest was reported by the authors(s).

Data availability statement

The genome sequence data that support the findings of this study are openly available in Genbank of NCBI at https://www.ncbi.nlm.nih.gov/under the accession no. MZ189977. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA743159, SRR15020542, and SAMN20000658, respectively.

Additional information

Funding

This work was supported by Beijing Municipal Natural Science Foundation [7202136], Projects of Sichuan Science and Technology Plan Projects [2019YFS0017], Nature Science Fund of Hebei Province, China [C2019408050] and National Program of Innovation Training for College Students [202010100003].